An improved method for the identification and quantitation of secondary bile acids in biological samples
β Scribed by Makoto Takahashi; Robert E. Raicht; Amar N. Sarwal; Erwin H. Mosbach; Bertram I. Cohen
- Publisher
- Elsevier Science
- Year
- 1978
- Tongue
- English
- Weight
- 563 KB
- Volume
- 87
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
## ABSTRACT In this work, a liquid chromatography coupled with electrospray ionization mass spectrometry (LCβESIβMS) method was developed and validated for quantification of bile acids in fecal materials. Coβeluting matrix impurities in fecal materials have been shown to greatly suppress the ioniza
A method is described for the analysis of GTP/GDP, or GDP alone, in a coupled enzymatic assay. Samples are pretreated with creatine kinase in the presence of phosphocreatine to remove the ADP present. Succinyl CoA synthetase is used to convert GTP to GDP in the presence of succinate and coenzyme A.
It has been suggested that pertmorooctanoic acid occurs in human plasma; however, no method of analysis for this compound in biological samples has been published to date. A method is presented for the analysis of perfluorooctanoic acid in plasma, urine, and liver tissue based on conversion of the a
The spectrophotometric periodate-resorcinol method for the measurement of free, bound and total N-acetylneuraminic acid has been modified. The proposed method improves sensitivity by 27%, reduces the time of analysis by 33-52% and addresses interferences from biological substances. The improved proc