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An improved method for the transformation of Lactobacillus strains using electroporation

✍ Scribed by Ming-Qian Wei; Catherine M. Rush; Julianne M. Norman; Louise M. Hafner; Ronald J. Epping; Peter Timms


Book ID
116107320
Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
939 KB
Volume
21
Category
Article
ISSN
0167-7012

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Pretreatment of yeast cells with lithium acetate (LiAc) and dithiothreitol (DTT) enhances the frequency of transformation by electroporation. The method shows improvements of 6-67-fold in wild-type strains derived from commonly used Saccharomyces cerevisiae genetic backgrounds. In addition, 15-300-f