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Optimization of technical conditions for the transformation of Lactobacillus acidophilus strains by electroporation

✍ Scribed by Y.H. Kim; K.S. Han; S. Oh; S. You; S.H. Kim


Book ID
108837907
Publisher
John Wiley and Sons
Year
2005
Tongue
English
Weight
192 KB
Volume
99
Category
Article
ISSN
1364-5072

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Pretreatment of yeast cells with lithium acetate (LiAc) and dithiothreitol (DTT) enhances the frequency of transformation by electroporation. The method shows improvements of 6-67-fold in wild-type strains derived from commonly used Saccharomyces cerevisiae genetic backgrounds. In addition, 15-300-f