A method for measuring inosine 5'-monophosphate (IMP) by enzymatic generation of NADPH is described. Procedures are given for direct fluorometric assay in the nanomole range and indirect measurement with amplification by enzymatic cycling in the pico- and femtomole ranges. The most sensitive procedu
An improved method for the enzymatic transformation of nucleosides into 5′-monophosphates
✍ Scribed by Vladimir N. Barai; Sergei V. Kvach; Anatoli I. Zinchenko; Igor A. Mikhailopulo
- Publisher
- Springer Netherlands
- Year
- 2004
- Tongue
- English
- Weight
- 79 KB
- Volume
- 26
- Category
- Article
- ISSN
- 0141-5492
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
A simple procedure to prepare a stable preparation of bovine liver adenosine kinase was developed. The adenosine kinase was used to phosphorylate a variety of purine nucleosides using ATP or (y-'\*P]ATP as the phosphoryl donor. A convenient scheme to purify the nucleotides was also developed. 0 1987
## Abstract For Abstract see ChemInform Abstract in Full Text.
Pyridoxal Y-phosphate was synthesized by first oxidizing [Wlpyridoxine to [Wlpyridoxal with MnOp ["C]Pyridoxal, isolated by cation-exchange chromatography, was then reacted with ATP in the presence of rat liver pyridoxal kinase to form ["C]pyridoxal Y-phosphate. The rat liver enzyme was used instead
2',3'-Unsaturated nucleosides have been prepared in excellent yields under mild conditions by the condensation of acetylated glycals with purine or pyrimidine derivatives in the presence of trityl perchlorate. The synthesis of ((w-and P-hex-Z enopyranosyl-4-ulose)theophylline nucleosides labelled at