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A preparative method for the enzymatic 5′-monophosphorylation of nucleosides

✍ Scribed by David J. Merkler; Vern L. Schramm

Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
487 KB
Volume
167
Category
Article
ISSN
0003-2697

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✦ Synopsis

A simple procedure to prepare a stable preparation of bovine liver adenosine kinase was developed. The adenosine kinase was used to phosphorylate a variety of purine nucleosides using ATP or (y-'*P]ATP as the phosphoryl donor. A convenient scheme to purify the nucleotides was also developed. 0 1987 Academic press, hc.

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