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An improved method for the isolation of supercoiled DNA molecules using ion-exchange column chromatography

✍ Scribed by Garon, Claude F; Petersen, Lori L

Book ID
123503320
Publisher
Elsevier Science
Year
1987
Weight
721 KB
Volume
4
Category
Article
ISSN
0735-0651

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An ion-exchange chromatographic procedure for the isolation of octopine from the mantle muscle of Sepia officinalis L. is described. The method is simple and reproducible, and the average yield is about 4.5 g of highly purified compound/kg of tissue. Some properties of octopine isolated from Sepia m