A highly sensitive method for quantification of sialic acids in gangliosides was developed. The sialic acids, released by hydrolysis of gangliosides, were converted to fluorescent derivatives with 1,2-diamino-4,5-(methylenedioxy)benzene (DMB) and separated on a reversed-phase C 18 column with an iso
An improved method for preparation of perbenzoylated ganglioside-derived sialic acids and nanogram detection of N-acetyl- and N-glycolylneuraminic acid by high performance liquid chromatography
✍ Scribed by Frank Unland; Johannes Müthing
- Publisher
- John Wiley and Sons
- Year
- 1992
- Tongue
- English
- Weight
- 563 KB
- Volume
- 6
- Category
- Article
- ISSN
- 0269-3879
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A simple, rapid, and extremely sensitive method for determining \(N\)-acetyl- and \(N\)-glycolylneuraminic acids in serum and in submandibular, sublingual, and parotid glands using high-performance liquid chromatography with a fluorometric detector is described. The neuraminic acids contained in the
Serum and tissue specimens from healthy Wistar rats and from rats with Walker 256 carcinoma were analysed for Nacetyl and N-glycolylneuraminic acid by high performance liquid chromatography (HPLC) as per-O-benzoylated derivatives. Both neuraminic acids were identified, while N-acetylneuraminic acid
An improved fluorometric HPLC method for sialic acid determination was developed by employing synthetic N-propionylneuraminic acid (NPNA) as an internal standard. A fixed amount of NPNA was added to a sialoglycoconjugate sample. After hydrolyzing sialioglycoconjugates with diluted sulfuric acid, the
On page 262, right column, in line 18, and on page 263 in line 5 of the legend to Fig. 2, the emission wavelength should read 448 nm instead of 488 nm. Also on page 263, in the legend to Fig. 2, line 2, the reaction temperature should read 60°C instead of 80°C.