An improved fluorometric HPLC method for sialic acid determination was developed by employing synthetic N-propionylneuraminic acid (NPNA) as an internal standard. A fixed amount of NPNA was added to a sialoglycoconjugate sample. After hydrolyzing sialioglycoconjugates with diluted sulfuric acid, the
✦ LIBER ✦
An Improved Fluorometric High-Performance Liquid Chromatography Method for Sialic Acid Determination: An Internal Standard Method and Its Application to Sialic Acid Analysis of Human Apolipoprotein E: Volume 300, Number 2 (2002), pages 260–266
✍ Scribed by Masaki Ito; Kiyoshi Ikeda; Yuki Suzuki; Kiyoshi Tanaka; Megumi Saito
- Publisher
- Elsevier Science
- Year
- 2002
- Tongue
- English
- Weight
- 7 KB
- Volume
- 304
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
On page 262, right column, in line 18, and on page 263 in line 5 of the legend to Fig. 2, the emission wavelength should read 448 nm instead of 488 nm. Also on page 263, in the legend to Fig. 2, line 2, the reaction temperature should read 60°C instead of 80°C.
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An Improved Fluorometric High-Performanc
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Masaki Ito; Kiyoshi Ikeda; Yuki Suzuki; Kiyoshi Tanaka; Megumi Saito
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Article
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2002
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Elsevier Science
🌐
English
⚖ 97 KB