Analysis of n-acetyl and n-glycolylneuraminic acid in rat serum and tissues with walker 256 carcinoma by high-performance liquid chromatography

✍ Scribed by E. Makatsori; A. Aletras; N. K. Karamanos; T. Tsegenidis

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 54 KB
• Volume: 13
• Category: Article
• ISSN: 0269-3879
• DOI: 10.1002/(sici)1099-0801(199902)13:1<57::aid-bmc815>3.0.co;2-8

✦ Synopsis

Serum and tissue specimens from healthy Wistar rats and from rats with Walker 256 carcinoma were analysed for Nacetyl and N-glycolylneuraminic acid by high performance liquid chromatography (HPLC) as per-O-benzoylated derivatives. Both neuraminic acids were identified, while N-acetylneuraminic acid was the predominant sialic acid. Samples from rats with generalized metastasis showed a significant increase (45-80%) of total sialic acids. This phenomenon in serum is caused by the overproduction of sialic acids, as a result of synthesis of both types of neuraminic acids to a similar molar ratio. The increase of sialic acids in rat bones with metastatic cancer is mainly because of increased N-acetylneuraminic acid synthesis. These results suggest that the molecular mechanisms responsible for cancer metastasis in different tissues may be closely associated with increased synthesis of dominating neuraminic acid.