𝔖 Bobbio Scriptorium
✦   LIBER   ✦

An improved method for assay of cholesterol 7α-hydroxylase activity

✍ Scribed by Tadashi Ogishima; Kyuichiro Okuda

Book ID
107714375
Publisher
Elsevier Science
Year
1986
Tongue
English
Weight
462 KB
Volume
158
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

A high-performance liquid chromatographi
A high-performance liquid chromatographic method for the assay of cholesterol 7α-hydroxylase activity
✍ Hiromitsu Yokohama; Issei Ohtsuka; Hiroyuki Shiojiri; Kouich Katayama; Seiji Ish 📂 Article 📅 1986 🏛 Elsevier Science 🌐 English ⚖ 379 KB

A high-performance liquid chromatographic method has been developed for the measurement of cholesterol 7cy-hydroxylase activity in liver microsomes. 7ol-Hydroxycholesterol generated from endogenous choleserol was derivatized with anthroyl l-c&o&rile, chromatogmphed on a revere phase column, and dete

Assay of cholesterol 7α-hydroxylase acti
Assay of cholesterol 7α-hydroxylase activity in rat hepatocytes in primary monolayer culture
✍ Hans M.G. Princen; Piet Meijer; Jaap Kwekkeboom; Herman Jan M. Kempen 📂 Article 📅 1988 🏛 Elsevier Science 🌐 English ⚖ 1009 KB

A sensitive and precise method is described to assay cholesterol 7 alpha-hydroxylase activity in homogenates of rat hepatocytes cultured in monolayers for up to 76 h. The assay is based on measurement of the amount of radioactive cholesterol converted into 7 alpha-[14C]-hydroxycholesterol. Since no