๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

An improved bulk purification method for Escherichia coli elongation factor, Ts

โœ Scribed by Marilyn Yoder; Joyce Lennane; Frances Jurnak

Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
560 KB
Volume
141
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

โœฆ Synopsis

A bulk purification procedure has been designed to maximize the yield of Escherichia coli elongation factor, Ts, with a minimum of effort and time. The enzyme purification is achieved by DEAE-Sepharose and elongation factor Tu-affinity chromatographies. The typical yield is 150 mg/kg of E. coli (B) cells.

๐Ÿ“œ SIMILAR VOLUMES

Bulk preparation and crystallization of
Bulk preparation and crystallization of the Escherichia coli elongation factor Tu-Ts complex
โœ Marilyn Yoder; Clare Tores; Robin Corelli; Frances Jurnak ๐Ÿ“‚ Article ๐Ÿ“… 1985 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 426 KB

A simple procedure for the preparation of lo-500 mg of the Escherichia coli elongation Tu-Ts complex is described. The protocol is based on the separate purification and quantitation of EF-Tu-GDP and EF-Ts, followed by mixing of equimolar amounts of each protein and removal of the displaced GDP by d

An improved method for the purification
An improved method for the purification of the nucleic acids from small amounts of Escherichia coli
โœ Allen C. Rogerson ๐Ÿ“‚ Article ๐Ÿ“… 1975 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 534 KB

An improved method for the rapid extraction and purification of the nucleic acids from small amounts of Escherichiu co/i is described. The method is applicable to multiple samples of about 5 X IO" cells each, and can be completed in about 4-6 hr. Recovery is on the order of 60%, there is little dete