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An improved boiling method for the preparation of bacterial plasmid and phage DNA

โœ Scribed by Ortlepp, Stephen A.

Book ID
121980124
Publisher
Elsevier Science
Year
1989
Weight
492 KB
Volume
6
Category
Article
ISSN
0735-0651

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A rapid boiling method for the preparati
A rapid boiling method for the preparation of bacterial plasmids
โœ David S. Holmes; Michael Quigley ๐Ÿ“‚ Article ๐Ÿ“… 1981 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 1005 KB

A simple and rapid method for preparing plasmids for restriction enzyme analysis has been developed. Bacteria are boiled for 15โ€“40 s and an insoluble clot of genomic DNA and debris is removed by low-speed centrifugation. Plasmids are recovered from the supernatant by isopropanol precipitation and ca