An improved assay and some properties of phosphoglycolate phosphatase

✍ Scribed by E. Beutler; C. West

• Publisher: Elsevier Science
• Year: 1980
• Tongue: English
• Weight: 468 KB
• Volume: 106
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(80)90132-3

✦ Synopsis

A method for the synthesis of [3ZP]phosphoglycolate is described; this substrate is used to assay phosphoglycolate phosphatase activity. This enzyme activity was demonstrated in several rat tissues. In human red cells, activity of the enzyme was age dependent. A correlation was found between 2,3-diphosphoglycerate levels and phosphoglycolate phosphatase activity in various species, suggesting that activity of this enzyme may have played some role in the evolutionary development of various mechanisms of regulation of the oxygen dissociation curve.