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An improved 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) reduction assay for evaluating the viability of Escherichia coli cells

โœ Scribed by Hengwei Wang; Hairong Cheng; Fengqing Wang; Dongzhi Wei; Xuedong Wang


Book ID
116750906
Publisher
Elsevier Science
Year
2010
Tongue
English
Weight
412 KB
Volume
82
Category
Article
ISSN
0167-7012

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A method to measure cell concentration in porous cellulose carriers was developed. Results of the comparison of 5 calorimetric assays showed that MlT(3-(4,5-dimethylthiazol-2-yl)-2.5diphenyl tetrazolium bromide) assay by the solubilization of the formazan with DMSO(dimethy1 sulfoxide) was the most s