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Ammonia-containing dimethyl sulfoxide: An improved solvent for the dissolution of formazan crystals in the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay

✍ Scribed by Hengwei Wang; Fengqing Wang; Xinyi Tao; Hairong Cheng


Book ID
113412305
Publisher
Elsevier Science
Year
2012
Tongue
English
Weight
459 KB
Volume
421
Category
Article
ISSN
0003-2697

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Improved MTT(3-(4,5-dimethylthiazol-2-yl
✍ Nakao Nomura; Kaoru Saijo; Mayako Kato; Pi-Chao Wang; Tadao Ohno; Masatoshi Mats 📂 Article 📅 1996 🏛 Springer-Verlag 🌐 English ⚖ 461 KB

A method to measure cell concentration in porous cellulose carriers was developed. Results of the comparison of 5 calorimetric assays showed that MlT(3-(4,5-dimethylthiazol-2-yl)-2.5diphenyl tetrazolium bromide) assay by the solubilization of the formazan with DMSO(dimethy1 sulfoxide) was the most s