An immunochemical method for determining thioredoxin reductase in rat tissues
β Scribed by Chin-Chun Chen; E.Colleen Moore
- Publisher
- Elsevier Science
- Year
- 1977
- Tongue
- English
- Weight
- 380 KB
- Volume
- 83
- Category
- Article
- ISSN
- 0003-2697
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β¦ Synopsis
The immunochemical method, microcomplement fixation, was used in the assay of thioredoxin reductase. The method is more sensitive and specific than previously used methods. The enzyme in different tissues of rats was measured.
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A specific and sensitive electron-capture gas-liquid chromatographic method was devised for the estimation of piperidine, a putative neurotransmitter, in rat and dog brain tissue. The procedure involves the addition of an internal standard, 2-ethylpiperidine, to brain homogenates followed by extract
## Methods Reagents. Bz-Arg-NPhNO, was obtained from Seikagaku Kogyo Co., Ltd. Phenylmethylsulfonyl fluoride was purchased from Sigma. All other chemicals were reagent grade and commercially available. p-Dimethylaminobenzaldehyde reagent was prepared as follows: Concentrated sulfuric acid, 2.74 ml