An enzyme immunoassay system for aflatoxin B1
โ Scribed by A. A. Burkin; G. P. Kononenko; N. A. Soboleva; E. V. Zotova
- Book ID
- 110658052
- Publisher
- SP MAIK Nauka/Interperiodica
- Year
- 2000
- Tongue
- English
- Weight
- 438 KB
- Volume
- 36
- Category
- Article
- ISSN
- 0003-6838
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๐ SIMILAR VOLUMES
A sensitive and specific enzyme immunoassay for secretin was developed with the use of enzyme-labeled antigens. Synthetic porcine secretin and its carboxy-terminal fragments (residues 11-27 and 18-27) were conjugated with beta-D-galactosidase for use in the immunoassay, and the assay method with the
Didemnin A was conjugated at the amino terminus of the N-methylleucine residue, via the linkers N-succinimidyl-3-(2-pyridyldithio)propionate and trans-l,4-maleimidomethylcyclohexane carboxylic acid, to keyhole limpet hemocyanin (KLH) and bovine serum albumin (BSA). The didemnin-KLH conjugates were u