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An enzyme immunoassay for determining plasma concentrations of didemnin B

✍ Scribed by T. J. G. Raybould; P. G. Grothaus; Samantha B. Simpson; G. S. Bignami; Carolyn B. Lazo; R. A. Newman

Publisher: John Wiley and Sons
Year: 1992
Tongue: English
Weight: 689 KB
Volume: 6
Category: Article
ISSN: 0887-8013
DOI: 10.1002/jcla.1860060307

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✦ Synopsis

Didemnin A was conjugated at the amino terminus of the N-methylleucine residue, via the linkers N-succinimidyl-3-(2-pyridyldithio)propionate and trans-l,4-maleimidomethylcyclohexane carboxylic acid, to keyhole limpet hemocyanin (KLH) and bovine serum albumin (BSA). The didemnin-KLH conjugates were used to hyperimmunize rabbits. The resulting high titer antisera were employed with didemnin-BSA conjugate-coated microtiter plate wells to develop an indirect competitive inhibition enzyme immunoassay (CIEIA) that was fully cross reactive with didemnin 6. A ClElA is described that is capable of detecting the drug in plasma from didemnin 6treated patients at concentrations down to 1-3 ng/ml. This simple, sensitive ClElA has been employed to demonstrate plasma drug clearance profiles with samples from didemnin 6-treated patients. o 1992 Wiley-Liss, tnc.

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