Development of an enzyme immunoassay for the determination of porcine growth hormone in plasma

✍ Scribed by B. Serpek; F. Elsaesser; H.H.D. Meyer

• Publisher: Elsevier Science
• Year: 1993
• Tongue: English
• Weight: 468 KB
• Volume: 275
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(93)80290-2

✦ Synopsis

A competltwe enzyme unmunoassay for the determmatlon of porcme growth hormone (pGH) was developed The assay IS based on (ant+rabbit IgQ sheep IgG-coated nucrot&e plates, antl-pGH from rabbits, blotmyl-pGH and streptamdm-horseradrsh peroxtdase The assay IS done dx&ly pvlth 40 ~1 of plasma and the cahbration graph (90% relative bmdmg at 0.36 ng ml-' and 50% relative bmdmg at 4 4 ng ml-') 1 prepared m plasma mthout measurable endogenous pGH Recovery of pGH added to dtfferent plasma samples amounted to 102-119%, and all vanab&es were < 8 6% The aa~y shows 50% cro~reaction with recombmant pGH, but no s&uuficant bmdmg of ponxne pro&m, folhcle stnnulatmg hormone and h~teunzmg hormone The assay detects htgh and low plasma pGH levels wtthm the pbyrnologtcal vanatton as well as changes m plasma pGH after s~ulatxm with gmwth hormone releasmg factor or treatment wrth recombmant pGH Hence It offers a rehable altematwe to radmmunoassay However, a reference method prowdmg wrdence on the Identity of exogenous pGH anal be. reqmred for provmg tiegal treatment wttb pGH and for forensrc purposes &ywvrd9 Enzymatuz methods, Immunoassay Plasma, Porcme growth hormone