Multiple enzyme analysis with computer processing of data: I. An automatic method for determination of the relation between enzyme activity and protein concentration

✍ Scribed by D.B. Roodyn; N.G. Maroudas

• Publisher: Elsevier Science
• Year: 1968
• Tongue: English
• Weight: 533 KB
• Volume: 24
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(68)90157-7

✦ Synopsis

The techniques are illustrated by studies on ferricyanide reductases in yeast homogenates.

Yeast strains. These were kindly supplied by Dr. D. Wilkie of the Department of Botany, University College London. Preparation of homogenates. Yeast was grown to stationary phase at 30" in yeast extract/peptone/2%

glucose with vigorous shaking. The cells were washed three times with distilled water, and disrupted in 0.3 M mannitol, by shaking with glass beads in a Braun shaker (3).