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An enzymatic fluorimetric assay for myo-inositol

โœ Scribed by Leslie C. MacGregor; Franz M. Matschinsky

Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
633 KB
Volume
141
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

An enzymatic assay for my&nositol (MI) is described. The method is based on the oxidation of MI by NAD+dependent my&nositol dehydrogenase, coupled to reoxidation of NADH with oxalacetate and malate dehydrogenase. The resultant malate is measured fluorimetrically. Several variations of the assay are described for measuring MI in serum and in tissues in amounts ranging from 0.2 pmol to 8 nmol. Highest sensitivity is achieved by applying an oilwell technique for handling small dropjets, and by using the principle of enzymatic cycling. The potential of the technique is illustrated by MI measurements in several tissues of normal and diabetic rats and Chinese hamsters.

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