๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

A sensitive bioluminescence assay for myo-inositol

โœ Scribed by T.W. Gudermann; T.G. Cooper

Publisher
Elsevier Science
Year
1986
Tongue
English
Weight
389 KB
Volume
158
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

A specific, sensitive bioluminescence assay for my&msitol is described employing myo-inositol dehydrogenase linked to a commercial NADH bioluminescence kit. Optimum conditions providing a linear response over 4 orders of magnitude are presented with reproducibility of 6% CV and a sensitivity of 1 pmol. The assay is suitable for the measurement of inositol at low concentrations or in Small VOhII'IeS.

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