𝔖 Bobbio Scriptorium
✦   LIBER   ✦

An engineered epigenetic transgene switch in mammalian cells

✍ Scribed by Kramer, Beat P; Viretta, Alessandro Usseglio; Baba, Marie Daoud-El; Aubel, Dominique; Weber, Wilfried; Fussenegger, Martin


Book ID
109911206
Publisher
Nature Publishing Group
Year
2004
Tongue
English
Weight
166 KB
Volume
22
Category
Article
ISSN
1087-0156

No coin nor oath required. For personal study only.


πŸ“œ SIMILAR VOLUMES


Transgene expression of transfected supe
✍ Christof Maucksch; Alexander Bohla; Florian Hoffmann; Martin Schleef; Manish Kum πŸ“‚ Article πŸ“… 2009 πŸ› John Wiley and Sons 🌐 English βš– 271 KB

## Abstract ## Background Supercoiled topology of transfected plasmid DNA (pDNA) is critical for transgene expression in mammalian cells. In the present study, we analysed transgene expression of transfected supercoiled pDNA concatemers. ## Methods Jurkat T cells were transfected with a supercoi

Transgene expression of transfected supe
✍ Jean-Michel Escoffre; Elisabeth Bellard; Muriel Golzio; Justin TeissiΓ©; Marie-Pi πŸ“‚ Article πŸ“… 2009 πŸ› John Wiley and Sons 🌐 English βš– 130 KB

We read with interest the article by Maucksch et al. [1], who analysed transgene expression of transfected supercoiled 4.7 kb monomeric and 9.4 kb dimeric plasmid concatemer in Jurkat T cells using electroporation. The authors reported that the relative number of electrotransferred gene copies per n