An engineered epigenetic transgene switch in mammalian cells
β Scribed by Kramer, Beat P; Viretta, Alessandro Usseglio; Baba, Marie Daoud-El; Aubel, Dominique; Weber, Wilfried; Fussenegger, Martin
- Book ID
- 109911206
- Publisher
- Nature Publishing Group
- Year
- 2004
- Tongue
- English
- Weight
- 166 KB
- Volume
- 22
- Category
- Article
- ISSN
- 1087-0156
- DOI
- 10.1038/nbt980
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## Abstract ## Background Supercoiled topology of transfected plasmid DNA (pDNA) is critical for transgene expression in mammalian cells. In the present study, we analysed transgene expression of transfected supercoiled pDNA concatemers. ## Methods Jurkat T cells were transfected with a supercoi
We read with interest the article by Maucksch et al. [1], who analysed transgene expression of transfected supercoiled 4.7 kb monomeric and 9.4 kb dimeric plasmid concatemer in Jurkat T cells using electroporation. The authors reported that the relative number of electrotransferred gene copies per n