An efficient algorithm for sequencing peptides using fast atom bombardment mass spectral data
β Scribed by Marshall M. Siegel; Norman Bauman
- Publisher
- John Wiley and Sons
- Year
- 1988
- Tongue
- English
- Weight
- 817 KB
- Volume
- 15
- Category
- Article
- ISSN
- 1076-5174
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π SIMILAR VOLUMES
Oligopeptides subjected to partial enzyme hydrolysis provide mixtures of sub-peptides which optimize amino acid sequence determination by fast atom bombardment mass spectrometry. The digestion mixture can be sampled directly; there is no interference from the enzyme. Complete sequences were obtained
Ten peptides containing one, two or three disulfides were examined to determine their behavior under fast atom bombardment (FAB) mass spectrometric conditions. The mass spectra for the disulfide and the reduced disulfide forms of each peptide were compared. Several factors were examined that contrib