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The combined use of enzymatic hydrolysis and fast atom bombardment mass spectrometry for peptide sequencing

✍ Scribed by R. Self; A. Parente

Publisher: John Wiley and Sons
Year: 1983
Tongue: English
Weight: 440 KB
Volume: 10
Category: Article
ISSN: 1076-5174
DOI: 10.1002/bms.1200100206

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✦ Synopsis

Oligopeptides subjected to partial enzyme hydrolysis provide mixtures of sub-peptides which optimize amino acid sequence determination by fast atom bombardment mass spectrometry. The digestion mixture can be sampled directly; there is no interference from the enzyme. Complete sequences were obtained from spectra recorded on 1-5 nmol samples after digestion at 37OC for times ranging from 0.14.0 h. The qualitative results of attack by two exopeptidases on three oligopeptides are discussed.

Abbreviations: CPY = carboxy peptidase Y; LAP-M = microsomal leucine aminopeptidase.

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