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Peptide studies using a fast atom bombardment high field mass spectrometer and data system. 4. Disulfide-containing peptides

✍ Scribed by Alexander M. Buko; Blair A. Fraser

Publisher
John Wiley and Sons
Year
1985
Tongue
English
Volume
12
Category
Article
ISSN
1076-5174

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✦ Synopsis

Ten peptides containing one, two or three disulfides were examined to determine their behavior under fast atom bombardment (FAB) mass spectrometric conditions. The mass spectra for the disulfide and the reduced disulfide forms of each peptide were compared. Several factors were examined that contribute to the fast atom bombardment mass spectra of these peptides: components of the FAB matrix such as alkali cations, acids, bases and reducing agents, the intrinsic molecular properties of the intact peptide, and the effect of reducing conditions on sensitivity. The FAB mass spectra of the disulfide-containing peptides examined in this study displayed accurate molecular weight information and fragmentation which indicated the position of the disulfide in the amino acid sequence.

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Peptide studies using a fast atom bombar
Peptide studies using a fast atom bombardment high field mass spectrometer and data system. 1β€”Sample introduction, data acquisition and mass calibration
✍ Alexander M. Buko; Lawrence R. Phillips; Blair A. Fraser πŸ“‚ Article πŸ“… 1983 πŸ› John Wiley and Sons 🌐 English βš– 952 KB

Conditions were established for analyzing as little as 5 pmol of an underivatized peptide delivered in a glycerol sample matrix as a thin film onto a gold-plated copper sample stage and then bombarded with xenon fast atoms. Calibration of the fast atom bombardment high field mass spectrometer and da