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An Easy and Reliable Procedure of Microdissection Technique for the Analysis of Chromosomal Breakpoints and Marker Chromosomes

✍ Scribed by Jörg Weimer; Marion Kiechle; Gabriele Senger; Ute Wiedemann; Angela Ovens-Raeder; Stefan Schuierer; Monika Kautza; Reiner Siebert; Norbert Arnold


Book ID
110273337
Publisher
Springer
Year
1999
Tongue
English
Weight
130 KB
Volume
7
Category
Article
ISSN
0967-3849

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A region-specific library for human chromosome 2p23-p25 was constructed using microdissection and polymerase chain reaction (PCR)-mediated microcloning techniques. This library is large, comprising 300,000 recombinant microclones. The insert sizes range between 50-600 base pairs (bp) with a mean of