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An improvement in the procedure for in situ hybridization of nuclei and chromosomes with digoxigenin-labelled probes

✍ Scribed by K.S. Jayarama; Ananya Bhattacharya; H. Sharat Chandra


Book ID
114392980
Publisher
Elsevier
Year
1997
Weight
166 KB
Volume
2
Category
Article
ISSN
1366-2120

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Messenger RNAs (mRNAs) encoding procollagen cd type I, cd type II and cd type III have been localized in paraffin sections of human fetal fingers using digoxigenin-labelled synthetic oligonucleotide probes. The probe-mRNA hybrids were visualized using an anti-digoxin antibody amplified with sandwich