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An assay for PEP carboxykinase in crude tissue extracts

โœ Scribed by M.D. Hatch

Publisher
Elsevier Science
Year
1973
Tongue
English
Weight
339 KB
Volume
52
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

A simple procedure is described for assaying phosphoenolpyruvate carboxykinase in the direction of oxaloacetate decarboxylation by following the decrease in oxaloacetate absorbance. The procedure offers a means of measuring the activity of this enzyme in its physiological direction in unfractionated tissue extracts. Phosphoenolpyruvate (PEP) carboxykinase catalyses the following reversible reaction: 289

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