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A simple spectrophotometric assay for fumarate hydratase in crude tissue extracts

โœ Scribed by M.D. Hatch

Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
333 KB
Volume
85
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

A procedure is described for assaying fumarate hydratase by coupling malate formed from fumarate to NADP+ reduction via NADP malic enzyme. The procedure is much more sensitive than existing assay methods and circumvents problems particularly associated with the use of these methods for determining fumarate hydratase in crude tissue extracts.

Methods

Biochemicals and reagent enzymes, including pigeon liver NADP malic enzyme [malate dehydrogenase (decarboxylating), (NADP+), EC1 . 1.1.40], were obtained from Sigma Chemical Co. (St. Louis, MO). Partially purified Zea mays (corn) leaf NADP malic enzyme, freed of fumarate hydratase

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