An assay for paracetamol, produced by the O-deethylation of phenacetin in vitro, using gas chromatography/electron capture negative ion chemical ionization mass spectrometry

✍ Scribed by S. Murray; A. R. Boobis

• Publisher: John Wiley and Sons
• Year: 1986
• Tongue: English
• Weight: 267 KB
• Volume: 13
• Category: Article
• ISSN: 1076-5174
• DOI: 10.1002/bms.1200130208

✦ Synopsis

A sensitive gas chromatographichass spectrometric assay for paracetamol, produced by the 0-deethylation of phenacetin by microsomal fractions of human liver, has been developed. The method uses the 3,5-bistrifluoromethylbenzoyl derivative of paracetamol and electron capture negative ion chemical ionization. This derivative has a negative ion mass spectrum which contains only a molecular ion at m / z 391 and, when this ion is specifically monitored, an amount of derivative equivalent to 1 pg of paracetamol can be detected. ('H,)Paracetamol was available for use as an internal standard and this allowed the development of an assay with a precision of 8% (SD) at 0.5 ng paracetamol per microsomal incubation and a lower limit of quantitative determination of 0.2 ng paracetamol per microsomal incubation.