An assay for 2-amino-3,8-dimethylimidazo[4,5-ƒ]-quinoxaline and 2-amino-3,4,8-trimethylimidazo-[4,5-ƒ]quinoxaline in fried beef using capillary column gas chromatography electron capture negative Ion chemical ionization mass spectrometry
✍ Scribed by S. Murray; N. J. Gooderham; A. R. Boobis; D. S. Davies
- Publisher
- John Wiley and Sons
- Year
- 1988
- Tongue
- English
- Weight
- 322 KB
- Volume
- 16
- Category
- Article
- ISSN
- 1076-5174
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✦ Synopsis
A gas chromatographic/mass spectrometric assay has been developed for the simultaneous measurement of Z ami~3,gdimethylimiidPzoM5.flquinoxal (MeIQx) and 2-amino-3,4,8-trimetbylimidazo[4,5--]quinoxaline (DiMeIQx) in fried beef. The method employs capillary column gas chromatography, electron capture negative ion chemical ionization mass spectrometry and a stable isotope labelled analogw of MeIQx as common internal standard. Two patties of lean minced beef which had been cooked separately were analysed and found to contain both compounds (patty 1: 2.4 ng MeIQx g-' meat, 1.2 ng DiMeIQx g-' meat; patty 2: 1.3 ng MeIQx g-' meat, 0.5 ng DiMeIQx g-' meat). Neither compound was present in the meat prior to cooking.