Ao amperometric mediated sensor for glucose has been contrived by using bovine serum albumin and glutaraldehyde to immobilize glucose oxidase on a Nafion-tetrathiafulvalene (TTF) modified electrode. It is further coated by Nafion. The inner Nafion membrane can prevent leaking of tetrathiafulvalene;
An amperometric lactate sensor employing tetrathiafulvalene in Nafion film as electron shuttle
โ Scribed by Haiying Liu; Jiaqi Deng
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 411 KB
- Volume
- 40
- Category
- Article
- ISSN
- 0013-4686
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โฆ Synopsis
The development of an amperometric sensor utilizing tetrathiafulvalene (TTF) as an electron shuttle between immobilized lactate oxidase and a glassy carbon electrode is described. The problem of mediator leaking in electrodes using TTF is overcome by employing Nafion membrane that prevents leaching of positive TTF+ ions by electrostatic attraction. Stability, interference, and the factors such as oxygen, applied potential, and pH influencing the performance of the lactate sensor are examined and discussed. The response of the sensor to lactate under N, saturation reaches 95% steady-state current within 40 s.
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The structural changes of the regenerated silk fibroin membranes for immobilizing glucose oxidase were characterized with FT-IR spectra. The results of electronic absorption spectra and scanning electron microscopy illustrated that the glucose oxidase in the membranes existed in aggretates. The blen