The structural changes of the regenerated silk fibroin membranes for immobilizing glucose oxidase were characterized with FT-IR spectra. The results of electronic absorption spectra and scanning electron microscopy illustrated that the glucose oxidase in the membranes existed in aggretates. The blen
โฆ LIBER โฆ
Characterization of regenerated silk fibroin membrane for immobilizing peroxidase and construction of an amperometric hydrogen peroxide sensor employing phenazine methosulphate as electron shuttle
โ Scribed by Jianghong Qian; Yongcheng Liu; Haiying Liu; Tongyin Yu; Jiaqi Deng
- Publisher
- Elsevier
- Year
- 1995
- Tongue
- English
- Weight
- 640 KB
- Volume
- 397
- Category
- Article
- ISSN
- 1572-6657
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A new composite membrane of poly(viny1 alcohol) (PVA) and regenerated silk fibroin (RSF) was successfully employed to immobilize horseradish peroxidase (HRP) and infrared (IR) was used to get insight in the structure of the composite membrane. An amperometric HRP-based sensor highly sensitive to hyd