The development of an amperometric sensor utilizing tetrathiafulvalene (TTF) as an electron shuttle between immobilized lactate oxidase and a glassy carbon electrode is described. The problem of mediator leaking in electrodes using TTF is overcome by employing Nafion membrane that prevents leaching
Amperometric glucose sensor using tetrathiafulvalene in Nafion gel as electron shuttle
✍ Scribed by Liu Haiying; Deng Jiaqi
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 550 KB
- Volume
- 300
- Category
- Article
- ISSN
- 0003-2670
No coin nor oath required. For personal study only.
✦ Synopsis
Ao amperometric mediated sensor for glucose has been contrived by using bovine serum albumin and glutaraldehyde to immobilize glucose oxidase on a Nafion-tetrathiafulvalene (TTF) modified electrode. It is further coated by Nafion. The inner Nafion membrane can prevent leaking of tetrathiafulvalene; the outer Nafion film serves as a barrier to electroactive anionic interferents such as ascorbate and urate and protects the biosensor from fouling agents. The experiment shows that lTF+ and 'lTF'+ can oxidize the reduced flavin adenine dinucleotide (FADH,) of glucose oxidase. The biosensor responds to glucose in less than 50 s and its calibration curve is linear from 3.0 X 10m4 to 1.0X lo-* M.
📜 SIMILAR VOLUMES
The structural changes of the regenerated silk fibroin membranes for immobilizing glucose oxidase were characterized with FT-IR spectra. The results of electronic absorption spectra and scanning electron microscopy illustrated that the glucose oxidase in the membranes existed in aggretates. The blen