An alkaline unwinding assay for the detection of DNA damage in aquatic organisms
β Scribed by Lee Shugart
- Book ID
- 119068087
- Publisher
- Elsevier Science
- Year
- 1988
- Tongue
- English
- Weight
- 239 KB
- Volume
- 24
- Category
- Article
- ISSN
- 0141-1136
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
An in vivo-in vitro alkaline DNA unwinding assay for single-strand breaks and/or alkalilabile lesions in hepatic DNA is described. The assay involves isolation of hepatic nuclei from mice, alkaline denaturation and unwinding of hepatic DNA, separation of single-and doublestranded DNA by hydroxylapat
The frequency of single-strand breaks in parental DNA and gaps in nascent DNA in various cells exposed to methyl methanesulfonate (MMS) or methylnitrosourea (MNU) was investigated by alkaline unwinding assay using two types of alkaline lysis conditions, 22Β°C lysis versus OΒ°C lysis. The DNA damage in
This procedure is to be used to detect the extent of DNA damage in tissues and body fluids of laboratory and environmentally exposed fish and mussels due to their exposure to environmental genotoxins. ## SCOPE This procedure is applicable to the following: 1. Aquatic biota, i.e., fish, mussels, c