DNA alkaline unwinding assay for monitoring the impact of environmental genotoxins

✍ Scribed by Rao, S. S. ;Nehell, T. A. ;Carey, J. H. ;Herbert, Armin ;Hansen, P. D.

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 261 KB
• Volume: 11
• Category: Article
• ISSN: 1053-4725
• DOI: 10.1002/(sici)1098-2256(1996)11:4<351::aid-tox10>3.0.co;2-c

✦ Synopsis

This procedure is to be used to detect the extent of DNA damage in tissues and body fluids of laboratory and environmentally exposed fish and mussels due to their exposure to environmental genotoxins.

SCOPE

This procedure is applicable to the following: 1. Aquatic biota, i.e., fish, mussels, clams, etc., exposed to contaminated receiving waters or in laboratory studies. The following materials can be used, i.e., fish livers, mussel tissues, blood, and hemolymph. 2. The DNA alkaline unwinding assay is a valuable environmental effects monitoring (EEM) tool for the detection and the assessment of the impact of environmental genotoxins and carcinogens at the organism level.