The frequency of single-strand breaks in parental DNA and gaps in nascent DNA in various cells exposed to methyl methanesulfonate (MMS) or methylnitrosourea (MNU) was investigated by alkaline unwinding assay using two types of alkaline lysis conditions, 22ยฐC lysis versus OยฐC lysis. The DNA damage in
Application of the DNA alkaline unwinding assay to detect DNA strand breaks in marine bivalves
โ Scribed by Diane Nacci; Sara Nelson; William Nelson; Eugene Jackim
- Book ID
- 119068394
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 898 KB
- Volume
- 33
- Category
- Article
- ISSN
- 0141-1136
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
## Abstract Strand breaks in hamster lung DNA were analyzed following __in vivo__ exposure to bleomycin. An alkaline unwinding procedure involving separation of singleโstrand from doubleโstrand DNA by hydroxylapatite chromatography followed by fluorescence detection of DNA with bisbenzimide was ada
## Abstract ## Background Formation and repair of DNA single-strand breaks are important parameters in the assessment of DNA damage and repair occurring in live cells. The 'Fluorimetric Detection of Alkaline DNA Unwinding (FADU)' method [Birnboim HC, Jevcak JJ. Cancer Res (1981) 41:1889โ1892] is a