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Amplification of λ plasmids in Escherichia coli relA mutants

✍ Scribed by Grzegorz Wȩgrzyn

Book ID
119132262
Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
539 KB
Volume
43
Category
Article
ISSN
0168-1656

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Amino acid starved cells of an E. coli relA strain accumulate a large amount of pBR322 plasmid DNA. In this study ColEl related plasmids of different copy number and size including a high copy number plasmid mutant of pBR322 were amplified in a relA strain of E. coli K-12 under amino acid limitation

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## Abstract Fermenter studies under batch and fed‐batch conditions were carried out to test the possibility of plasmid pBR322 production in large amounts by using __E. coli relA__ strains. High amplification rates of pBR322 plasmid DNA were observed in __E. coli__ CP79 (__relA__) and __E. coli__ CP