✦ LIBER ✦

Amplification of the amyE-tmrB region on the chromosome in tunicamycin-resistant cells of Bacillus subtilis

✍ Scribed by Hashiguchi, Ken-ichi ;Tanimoto, Akihiko ;Nomura, Shintaro ;Yamane, Kunio ;Yoda, Koji ;Harada, Shigeyoshi ;Mori, Masaki ;Furusato, Takashi ;Takatsuki, Akira ;Yamasaki, Makari ;Tamura, Gakuzo

Publisher: Springer
Year: 1986
Tongue: English
Weight: 801 KB
Volume: 204
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00330184

No coin nor oath required. For personal study only.

✦ Synopsis

In a class of tunicamycin-resistant mutants (tmrA7) of Bacillus subtilis, the production of extracellular alpha-amylase is increased by about five fold. The tmrA7 characteristics (tunicamycin resistance and hyperproduction of extracellular alpha-amylase) can be transferred to recipient cells by transformation. In the transformants and the original tmrA7 mutant, typical amplification of the region from 4 kb upstream of the amyE gene to the tmrB gene on the chromosome was detected. The repeating unit, 16 kb in size, repeats tandemly about five and ten times in the mutant and transformants, respectively, and the alpha-amylase production is proportional to the copy number of the amyE gene. Simultaneous amplification of the tmrB gene, which is responsible for tunicamycin resistance in the multicopy state, and the alpha-amylase structural gene (amyE) seems to be the cause of the pleiotropy of the tmrA7 mutation.

📜 SIMILAR VOLUMES

Genetic analysis of a class of polymyxin

Genetic analysis of a class of polymyxin resistant partial revertants of stage O sporulation mutants of Bacillus subtilis: Map of the chromosome region near the origin of replication

✍ Trowsdale, John ;Chen, Shu Mei H. ;Hoch, James A. 📂 Article 📅 1979 🏛 Springer 🌐 English ⚖ 818 KB

Amplification of the Escherichia coli la

Amplification of the Escherichia coli lacZ gene in Bacillus subtilis and its expression on a by-product growth medium

✍ Ehab R. El-Helow; Khaled M. Ghanem; Eman A. Mohamad 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 117 KB 👁 2 views

Detection of 11q13 amplification as the

Detection of 11q13 amplification as the origin of a homogeneously staining region in small cell lung cancer by chromosome microdissection

✍ Jia Xu; Tina Tyan; Edward Cedrone; Niramol Savaraj; Nancy Wang 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 640 KB

Chromosomal homogeneous staining region (hsr), which is a cytogenetic indication of gene amplification, was found in the pleural effusion (BHII) of a patient with small cell lung cancer (SCLC) after failure of multiple drug treatments. Amplification of band I I q I 3 was identified as the origin of

Modification of length, hydrophobic prop

Modification of length, hydrophobic properties and electric charge of Bacillus subtilis α-amylase signal peptide and their different effects on the production of secretory proteins in B. subtilis and Escherichia coli cells

✍ Nakamura, Kouji ;Fujita, Yasuhito ;Itoh, Yoshiki ;Yamane, Kunio 📂 Article 📅 1989 🏛 Springer 🌐 English ⚖ 977 KB

Amplification on double-minute chromosom

Amplification on double-minute chromosomes and partial-tandem duplication of the MILL gene in leukemic cells of a patient with acute myelogenous leukemia

✍ Yoji Ariyama; Yoji Fukuda; Yoshiaki Okuno; Masao Seto; Kose Date; Tatsuo Abe; Yu 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 588 KB

Amplification of the MLL gene on double

Amplification of the MLL gene on double minutes, a homogeneously staining region, and ring chromosomes in five patients with acute myeloid leukemia or myelodysplastic syndrome

✍ Berthold Streubel; Peter Valent; Ulrich Jäger; Martin Edelhäuser; Hannes Wandt; 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 184 KB 👁 2 views

Gene amplification is one of the mechanisms for activating proto-oncogenes resulting in an enhanced expression of the corresponding gene product. By fluorescence in situ hybridization (FISH), amplification of the proto-oncogene MLL has been described only in seven patients with acute myeloid leukemi