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Amplification and overexpression of the epidermal growth factor receptor gene in human renal-cell carcinoma

✍ Scribed by Jiro Ishikawa; Sakan Maeda; Kei-Ichi Umezu; Taketoshi Sugiyama; Sadao Kamidono

Publisher
John Wiley and Sons
Year
1990
Tongue
French
Weight
512 KB
Volume
45
Category
Article
ISSN
0020-7136

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✦ Synopsis

We examined 22 cases of renal-cell carcinoma (RCC) for structural alterations of the epidermal growth factor receptor (ECFR) gene and found gene amplification in one case of high-stag+high-grade RCC. Dot blot analysis of the total RNA from tumorous and normal kidney tissues revealed overexpression of the EGFR gene in I 2 of 20 (60%) cases of RCC. The highest expression was observed in the geneamplified case. No correlation was observed between the level of EGFR mRNA and tumor stage or grade. Northern blot analysis revealed normal 10and 5.6-kb ECFR mRNA bands in RCC. Our data indicate that gene amplification of the ECFR gene is one of the molecular mechanisms of its overexpression in a subset of RCCs.

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