AML and Ets proteins regulate the Iα1 germ-line promoter

✍ Scribed by Xiao-Qi Xie; Evangelia Pardali; Magnus Holm; Paschalis Sideras; Thomas Grundström

• Book ID: 101309983
• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 253 KB
• Volume: 29
• Category: Article
• ISSN: 0014-2980
• DOI: 10.1002/(sici)1521-4141(199902)29:02<488::aid-immu488>3.0.co;2-n

✦ Synopsis

The immunoglobulin heavy chain (IgH) class switch recombination of B lymphocytes preferentially targets unrearranged IgH genes that have already been rendered transcriptionally active. Transcription of the germ-line IgH genes is controlled by intervening (I) regions upstream of their switch regions. The I alpha1 promoter activates transcription of the human germ-line C alpha1 gene for IgA1 and mediates the transforming growth factor (TGF)-beta1 responsiveness of this locus. Here we show that the I alpha1 promoter contains several binding sites for the AML/PEBP2/CBF family of transcription factors and that AML and Ets proteins are major regulators of the basal and TGF-beta-inducible promoter activity. Our data constitute a starting point for studies to elucidate the molecular mechanism by which TGF-beta regulates IgA production.