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Aminoacyl-tRNA synthetases

โœ Scribed by Marc Delarue


Book ID
116165974
Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
881 KB
Volume
5
Category
Article
ISSN
0959-440X

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Aminoacyl-tRNA synthetases
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Colorimetric assay for aminoacyl-tRNA sy
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A simple, rapid, and inexpensive procedure for the determination of aminoacyl-tRNA synthetase activity is described. The assay is based on a green color formed between PPi and ammonium molybdate in the presence of mercaptoethanol. The sensitivity is in the nanomole range and is comparable with the c