Amino-acid Type Identification in15N-HSQC Spectra by Combinatorial Selective15N-labelling
β Scribed by Peter S. C. Wu; Kiyoshi Ozawa; Slobodan Jergic; Xun-Cheng Su; Nicholas E. Dixon; Gottfried Otting
- Book ID
- 106401226
- Publisher
- Springer Netherlands
- Year
- 2006
- Tongue
- English
- Weight
- 815 KB
- Volume
- 34
- Category
- Article
- ISSN
- 0925-2738
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Amino acid type-selective triple-resonance experiments can be of great help for the assignment of protein spectra, since they help to remove ambiguities in either manual or automated assignment procedures. Here, modified triple-resonance experiments that yield amino acid type-selective 1 H-15 N corr
## Abstract Nuclear magnetic resonance spectra of membrane proteins containing multiple transmembrane helices have proven difficult to resolve due to the redundancy of aliphatic and Ser/Thr residues in transmembrane domains and the low chemical shift dispersity exhibited by residues in Ξ±βhelical st