Amino acid analysis : II. Ninhydrin reduction with titanous chloride
β Scribed by L.B. James
- Book ID
- 108334539
- Publisher
- Elsevier Science
- Year
- 1978
- Tongue
- English
- Weight
- 246 KB
- Volume
- 152
- Category
- Article
- ISSN
- 1873-3778
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π SIMILAR VOLUMES
The reaction with indane-1,2,3-trione-2-hydrate (ninhydrin) is firmly established for the calorimetric determination of a-amino acids (l-3). The intensity of the blue-violet color produced is measured at about 570 rnp. This color is most intense when the reaction with ninhydrin is carried out at abo
When potassium ferrocyanide is titrated with zinc ions, K2Zn3[Fe(CN)6]2 is formed only slowly and hence only one end-point is observed corresponding to a combining ratio of 2 : 1. In highly concentrated solutions of K4Fe(CN)6 the formation of K2Zna[Fe(CN)6]2 is rapid and hence a 1.5 : 1 ratio is obt
A procedure which allows quantitative determination of the amino acid composition of stained proteins separated by sodium dodecyl sulfate (SDS)-gel electrophoresis using ninhydrin is described. The Coomassie-protein-SDS complex extracted from the gel is dialyzed, lyophylized, and subjected to amino
equiv, depolarizer, the oxidation wave of hydroquinone attains its limiting height when four alkali hydroxide equivalents (NaOH or LiOH) are added to the solution. Since the wave is diffusion-controlled, a kinetic reaction is ruled out. It is probable that alkali hydroxides in pure non-aqueous metha