Amide Hydrogen Exchange in a Highly Denatured State: Hen Egg-white Lysozyme in Urea
✍ Scribed by Matthias Buck; Sheena E. Radford; Christopher M. Dobson
- Book ID
- 115626564
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 438 KB
- Volume
- 237
- Category
- Article
- ISSN
- 0022-2836
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## Abstract A molten globule‐like state of hen egg‐white lysozyme has been characterized in 25% aqueous hexafluoroacetone hydrate (HFA) by CD, fluorescence, NMR, and H/D exchange experiments. The far UV CD spectra of lysozyme in 25% HFA supports retention of native‐like secondary structure while th
## Abstract A first detailed comparison of the ^13^C spectra at high field of two lysozymes (human and egg‐white) and two α‐lactalbumins (human and bovine milk) is presented. Assignments were made on most of the resonance peaks in the aliphatic and aromatic regions of the denatured proteins. The re