Alternative splicing of the neurofibroma
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Takashi Koh; Jun Yokota; Keizo Ookawa; Tatsuo Kina; Kunio Koshimura; Soichi Miwa
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Article
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1995
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John Wiley and Sons
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French
β 1015 KB
The supernatant was gently removed and replaced by 2% BSA. This dish was incubated for another hour to block non-specific binding sites in the laminin, and washed twice with PBS, then 5 x lo5 cells were seeded on to each laminin-coated dish. ## RT-PCR analysis Total RNA was isolated from the cult