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Alternative Splicing of Human T-Cell-Specific MAL mRNA and Its Correlation with the Exon/Intron Organization of the Gene

✍ Scribed by Carmen Rancaño; Teresa Rubio; Miguel A. Alonso

Book ID: 115612154
Publisher: Elsevier Science
Year: 1994
Tongue: English
Weight: 243 KB
Volume: 21
Category: Article
ISSN: 0888-7543
DOI: 10.1006/geno.1994.1294

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The supernatant was gently removed and replaced by 2% BSA. This dish was incubated for another hour to block non-specific binding sites in the laminin, and washed twice with PBS, then 5 x lo5 cells were seeded on to each laminin-coated dish. ## RT-PCR analysis Total RNA was isolated from the cult