β Scribed by Takashi Koh; Jun Yokota; Keizo Ookawa; Tatsuo Kina; Kunio Koshimura; Soichi Miwa; Tetsuya Ariyasu; Hitoshi Yamada; Mitsuhiko Osaka; Hironori Haga; Shigeki Hitomi; Taketoshi Sugiyama; Rei Takahashi
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The supernatant was gently removed and replaced by 2% BSA. This dish was incubated for another hour to block non-specific binding sites in the laminin, and washed twice with PBS, then 5 x lo5 cells were seeded on to each laminin-coated dish.
Total RNA was isolated from the cultured cells of 15 human SCLC cell lines by the guanidinum thiocyanate method (Dee-'To whom correspondence and reprint requests should be sent, at
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Hel-NI and HuD belong to the elav gene family and have gained recent attention as potential neuroendocrine markers for small-cell lung carcinoma (SCLC). Members of this conserved family normally appear at different stages of neuronal maturation, raising the possibility that their expression patterns