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Alternative methods for genetic transformation of Pseudozyma antarctica, a basidiomycetous yeast-like fungus

✍ Scribed by G. Marchand; E. Fortier; B. Neveu; S. Bolduc; F. Belzile; R.R. Bélanger

Book ID
116750349
Publisher
Elsevier Science
Year
2007
Tongue
English
Weight
907 KB
Volume
70
Category
Article
ISSN
0167-7012

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Polyethylene glycol (PEG) can induce genetic transformation in both bacteria (Escherichia coli) and yeast (Saccharomyces cerevisiae) without cell wall removal. PEG-mediated transformation of E. coli is technically simple and yields transformants with an efficiency of 10(6)-10(7) transformants/microg